Validation and characterization of murine gammaherpesvirus 68 antisense transcripts by northern blot analysis and quantitative reverse transcription-PCR

The transcription of mammalian genomes exhibits an intriguing complexity and numerous novel RNA molecules have been identified. Viruses with large DNA genomes, especially herpesviruses, generate many different species, including long non-coding RNAs (lncRNAs). Dense viral can multigenic transcripts in addition to commonly observed antisense transcripts. It is essential study the biological roles these aside from protein-coding counterparts. Multiple open reading frame (ORF) 63-64 locus murine gammaherpesvirus 68 (MHV68) were detected by northern blotting. Expression analysis quantitative reverse PCR (qRT-PCR) did not detect isoforms. Several alternative splicing isoforms exist during lytic replication; however, they are latency. To identify new transcripts, qRT-PCR may be enough should supported method such as A more detailed transcriptional map interest useful design experimental strategies perform functional studies, when working gene-dense genomes.